Gelatin methacryloyl (GelMA) is a versatile biomaterial. The mechanical properties of GelMA hydrogels are closely related to the polymerization rate, which depends on the GelMA / photoinitiator ratio, light intensity, and exposure time. The control of the mechanical properties of the cross-linked gel begins in the formulation stage of the bio-links, varying the degree of functionalization and concentration of GelMA.
Impedance and temperature changes were measured in real-time during the light-curing process of Gelatin methacryloyl, a protein-based hydrogel used for various research purposes such as cell culture research.
For measurement, a sensor capable of simultaneously measuring impedance and temperature was fabricated using a semiconductor and microelectromechanical systems (MEMS) process.
Through temperature measurement, the endothermic reaction in the crosslinking of methacrylic anhydride (MAA), a crosslinking agent, was confirmed during the light-curing process.
In addition, it was confirmed that the impedance decreased due to charge transfer as photocuring proceeded between gelatin, PBS solvent, and polymer in the polymer formation process.
The impedance and the temperature changes of gelatin methacryloyl, a protein-based hydrogel used for various studies such as cell culture studies, were measured in real-time.
Sensors capable of simultaneously measuring the impedance and the temperature were fabricated using semiconductor and microelectromechanical systems (MEMS) processes.
The occurrence of an endothermic reaction in the crosslinking of methacrylic anhydride, a crosslinking agent, was confirmed by the measurement of the temperature. Also, the measured impedance monotonically decreased during the gelatin methacryloyl hydrogel process.
Gelatin methacryloyl is a material that chemically bonds gelatin with methacrylic anhydride. It is cross-linked by light to form a gel. It shows excellent cell adhesion and biocompatibility properties. Its physical properties can be controlled according to the degree of substitution of the methacrylate group and the concentration of the GelMA solution.